Bone marrow stem cells have been assessed to be capable of heart tissue regeneration and cardiac function recovery in both animals and human beings. Objective: To assess the effectiveness of separating stem cells from bone marrow with automatic machines; to identify some characteristics of stem cells blocks. Subject and method: 45 patients (40 males and 5 females) with heart failures after acute myocardial infarction, at the average age of 53.49 +/- 9.58, obtained the reperfusion coronary intervention success with percutaneous coronary arteries within the first 5 days after the first myocardial infarction; each patient was taken 250 ml of aspirate bone marrow from the pelvic bone and extracted components of mononuclear cells by automatic machines. Result: The final BMSC suspension volume was 10mI; the suspension cells concentration was 63.37 +/- 39.08 G/L with 60.27 +/- 13.86 percent of mononuclear cells; the CD 34+ cell concentration was 8.74 +/- 6.83 x 10 exponent 6; the mononuclear cell and CD 34+ cell recovery ratio was 23.69 +/- 12.65 percent and 47.59 +/- 24.42 percent respectively; the CD 34+ cell concentration increased by 15.28 +/- 7.94 times; the RBC and hemoglobin removing ratio was 89.21 percent. Conclusion: The process of collecting and separating stem cells from bone marrow by automatic machines was very effective.
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