Diabetes mellitus is characterized by abnormally high levels of glucose in the blood. The chronic hyperglycemia is caused by different reasons. Out of these reasons, enzyme glucose-6-phosphatase (G6Pase) which is mainly presented in the livers and kidneys taking an important role in controlling the blood glucose level. The objective is to estimate the activity of enzyme G6Pase in the livers and kidneys of diabetic mice treated with pandan (Pandanus amaryllifolius) ethanolic leafs extract. Mice were induced diabetes by intraperitoneal injection of alloxan monohydrate at concentration of 130 mg/kg body weight. After 7 days of diabetic incubation, mice having diabetes were orally administrated pandan leaf extract (400 mg/kg body weight x 2 times/day) or glucofast medicine (150 mg/kg body weight x 2 times/day). After 20 days of treatment, livers and kidneys of mice were used for determining the inhibitive ability on G6Pase. The results showed that pandan leaf's extract can significantly reduce the blood glucose concentration in diabetic mice after 20 days of treatment from 305.75 mg/dl to 125.5 mg/dl, a normal blood glucose level eventually. The inhibitive ability on G6Pase of ethanolic extract from pandan leaves on livers and kidneys of diabetic mice was dramatically effective. The results also indicated that livers and kidneys of untreated diabetic mice produced 8.3257 mg/I and 3.0858 mg/I inorganic phosphate which significantly differed from those of control mice with 3.5902 mg/I and 1.4258 mg/I, respectively. By treating the diabetic mice with ethanolic extract from pandan leaves, the inorganic phosphate produced in their livers and kidneys significantly decreased with 2.4914 mg/I and 0.4119 mg/l, repectively. In conclusion, the extract of pandan leaves showed the anti diabetic property by inhibiting glucose-6-phosphatase in vivo.
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