áp dụng kỹ thuật phản ứng chuỗi Polymerase (PCR) để phân biệt ấu trùng giun móc với ấu trùng giun mỏ tại một xã miền núi tỉnh Phú Thọ

The 33 fecal samples contained hookwormlike eggs were cultured for 7-10 days. After that the authors collected larvae and put in to 1.5ml tubes. Then DNA were extracted for using PCR method. Electraphoresis of specificaly amplified PCR products of A. duodenale and N. americanus from the eggs of each 690 bp and 870 bp respectively. The results of this study were shown that 28/33 cases were infected by sing N. americanus (contaened 84.84 percent) 5 cases were mix infected by A. duodenale and N. americanus (contaened 15.6 percent) non of any cases was infected by single A. duodenale.