Khảo sát mối liên quan liều, nồng độ tacrolimus, mức lọc cầu thận với đa hình kiểu gen CYP3A5 ở người bệnh ghép thận

To analyse the association of concentrations and dose of tacrolimus with CYP3A5 genetic polymorphism during 6 months after kidney transplantation. Subject and method: Eighty-eight kidney transplant recipients treated with tacrolimus, monitored during the first 6 months post transplantation at the 103 Military Hospital from 06/2017 to 10/2021. Blood samples were collected using for routine monitoring of therapeutic drug levels and determination of CYP3A5 genetic polymorphism based on inhouse molecular methods. Association of CYP3A5 genetic polymorphism with tacrolimus concentrations, doses, concentrations/doses ratio (C0/D) and eGFR (estimated glomerular filtration rate was investigated based on CYP3A5 genotypes at T1 (after trasplantation 1 month), T3 (after trasplantation 3 months), T6 (after trasplantation 6 months). Result: Frequency of CYP3A5*3*3, CYP3A5*1*3, and CYP3A5*1*1 genotypes were 43.2%, 46.6% and 10.2%, respectively. Patients with the CYP3A5*1*3, and CYP3A5*1*1 genotype had a lower C0, C0/D ratio and eGRF at T1, T3, T6, whereas daily dose of tacrolimus was higher at T1, T3 and T6, respectively as compared with those of CYP3A5*3*3. There was significant correlation of TAC doses and trough concentrations at corresponding time points of T1, T3 and T6 tested. Conclusion: The relation of CYP3A5 genetic polymorphism with trough concentrations, dose of tacrolimus and eGFR, provided a valuable tool for individualized dose of tacrolimus in renal transplant population in Vietnam.