Objective: To determine the presence of certain bacterial species and their numbers in necrotic root canals in three treatment phases: pre-shaping, post-shaping and after placing calcium hydroxide into root canals. Subject and method: 13 teeth diagnosed with pulp necrosis were treated and taken samples at Hanoi Central Hospital of Dentistry and No.108 Military Central Hospital during period from 11/2011 to 08/2014. Calcium hydroxide was placed in the root canal 7 days for disinfection before sealing the root canal system. Tests for bacteria were performed before treatment (51 phase); After shaping and irrigating of the canals (52 phase); After placing antiseptic drug into canals (53 phase). This was an uncontrolled, interventional studies. Result and conclucion: Species appeared most frequently in each treatment phase were as follow: Veillonella sp (84.6 percent), S.oralis, M.micros (61.5 percent) in 51 phase; S.oralis, S.sanguinis (38.5 percent), S.mitis, Veillonella sp (30.8 percent) in 52 phase; and S.sanguinis (30.8 percent) in 53 phase. Species had the highest average number of bacteria in positive samples in each treatment phase were: S.sanguinis ( 1.6 x 105 CFU/ml) in 51 phase; Prevotella oralis, E.faecalis, M.micros, S.oralis ( 1 x 105 CFU/ml) in 52 phase; and S.sanguinis (2 x 104 CFU/ml) in 53 phase.
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