Nghiên cứu xây dựng quy trình xác định tính đa hình gene IL28B và bước đầu khảo sát gene IL28B ở bệnh nhân viêm gan C

Introduction: Single nucleotide polymophisms (SNPs) near the gene IL28B is considered as the most important host factor that defines the success of Peg-INF alpha/ribavirin based regiment to eradicate HCV. Aim: i) To set-up a protocol to screen for rs8099917, rs12980275 and rs12979860 near IL28B gene ii) and statistcally record the epidemologics of IL28B SNP within healthy Vietnamese and Vietnamse HCV carriers. Subjects: genomics DNA sample from 32 healthy donor 38 HCV carriers. Methods: Allele specific kenetics real-time PCR, primers sets optimized for rs8099917, rs12980275 and rs12979860 SNP were designed; Sybr green 1 was used to record the florescent signal. Results: Allele specific kenetics real-time PCR was susessfully established that allow us to simutaneously detect all three inspected SNP in one run. the primarily data revealed 0/70 (0 percent) carries homozygous minor alleles; (90 percent), (79 percent), (79 percent) are homozygous major allele of rs8099917, rs12980275 and rs12979860, respectively. The percentage of heterogenous allele carriers are rs8099917 (70 percent), rs12980275 (21 percent), rs12979860 (21 percent). The authors also observed the frequency of rs8099917 major allele (T/T) in HCV carrier group is significantly higher than that in healthy control group (100 percent vs 79 percent, P 0,01), whereas there is no allelicfrequency difference of rs12980275 and rs 12979860 between the two study group. Conclusion: an income-appropriate-IL28B screen assay has recapitulated, which helped to record a statistically difference of SNP rs8099917, rs12980275, rs12979860 frequencies within Vietnamese health and HCV carriers.