Objective: To identify prevalence, genotype distribution of HCV among Drug Intravenous User (IDU) and Female Sex Worker (FSW) groups in Vietnam northern coastal area. Subject and methods: Plasma samples were collected from 1566 subjects among two groups (IDU: 1155; FSW' 411) in Vietnam northern coastal area. Plasma viral RNA was extracted from samples that were positive for anti-HCV. 5 'UTR-core va NS5B regions oj HCV were amplified by RT-PCR and Nested PCR. PCR products were sequenced directly or after cloning, and genotyped. Results and conclusions: Anti-HCV prevalence was 65,37 percent and 22,38 percent among IDU and FSW groups in Vietnam northern coastal area. Ten HCV genotypes/subtypes were confirmed. Subtypes 1a; 1b; 6a; 6e; 3b was predominant. the authors dint find out genotype 4; 5 and subtype 2; 6n; 6u among IDU and FSW groups. There is no significant difference of the HCV genotype distribution in each group of five provinces in Vietnam northern coastal area. Style of co-infection/recombination of HCV (6a/1a (23,53 percent); 1a/6a (11,77 percent); 6e/1a (11,77 percent); 6a/1b (8,82 percent); 6a/6e (8,82 percent)) were most common in Haiphong. The establishment of the HCV database as trend of HCV prevalence; genotype distribution; routes of HCV transmission among IDU and FSW would be needed for monitoring of hepatitis C in Vietnam northern coastal area
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