Tác động của chất chiết xuất từ dây rốn đến sự phát triển và biểu hiện gen của nguyên bào sợi da người nuôi cấy

Wrinkles are consequences of skin aging. This process is characterized with collagen breakdown and shortage in skin structure. This study tested the effects of newborn umbilical cord extracts on the proliferation and synthesis of collagen type 1, fibronectin and metalloproteinase 1 (MMP-1) of human skin fibroblast. 25 formulas made of 4 types of extracts included: extracellular extract of cord tissue; intracellular extract of cord cells; mixtures of these two extracts; intracellular extract of umbilical cord stem cells. were supplemented to cultUre media of human fibroblast. Cell proliferation was evaluated by MTT assay; gene expression was analyzed by Real-time RT-PCR technique. The results showed that supplementation of 7.5 percent of formula 3-5 containing a combination of both extra- and intra-cellular extracts of umbilical cord led to the highest cell proliferation and also stimulated the expression of collagen type 1 while inhibited. the expression of MMP-1 on fibroblast. These data suggested that umbilical cord extract would be a potential source of material for wrinkle reduction skin care product.