Real-time Quantitative Polymerase Chain Reaction (RQ-PCR) is the first technique of choice for detection of minimal residual disease in acute lymphoblastic leukemia (ALL) expressed fusion genes. In this research, the authors established conditions for TaqMan-based RQ-PCR of minor BCR/ABL (mBCR/ABL) and TEL/AML1 which are the most frequently occurring fusion genes in Blineage ALL (B-ALL). The results of this study showed that the standard curves of ABL, mBCR/ABL, and TEL/AML1 were set up successfully with R2 0.995, and PCR efficiency 95 percent. Establishing conditions for RQ-PCR of mBCR/ABL and TEL/AML1 fusion genes is the first step in standardizing minimal residual disease procedure in B-ALL at Blood Transfusion Hematology Hospital, Ho Chi Minh city.
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