A total of 532 married women at the age of child bearing 18-49 in Tam Nong - Phu Tho were gynecologically examinated and the samples of their vaginal discharge were taken to identify parasites. The results produced 93 Candida spp samples by PAP tests, Gram staining and culture in Saboraud Enviroment. Then all of them were tested by PCR technique, to identify fungus types. The results were follows: 69/93 (74.2 percent) ofthe samples were positive, 24 samples were negative (-). PCR with gene primers ITS1 5' TCC GTA GGT GAA CCT GCG G 3' and ITS4: 5' TCC TCC GCT TAT TGA TAT GC 3' is appropriate. The rate of fungus samples detected by PCR in Quang Huc, Te Le, and Tho Van was 19.2 percent, 26.3 percent and 31.8 percent respectively. Dimensions of the product having DNA cut by restriction enzymes produced the following results: Candida gabrata (C. glabrata) 557,314 bp; Candida tropical (C. tropical) 340 bp; Candida albicans (C. albicans) 297,238 bp; Candida krusei (C. krusei) 261,249 bp; Candida parapsilosis (C. parapsilosis) 176,243 bp. Rate and composition of fungal species in three communes was C. glabrata accounting for 43.5 percent, C. tropical occupying 34.8 percent, C. albicans holding 14.6 percent, C. krusei taking 4.3 percent, and C. parapsilosis possessing 2.8 percent.
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