Xây dựng quy trình PCR điện di phát hiện virus gây hội chứng viêm đường hô hấp cấp Trung đông

The Middle East respiratory syndrome is clinically similiar to the Severe Acute Respiratory Syndrome - SARS, but the tool to differentiate the two diseases is unavailable at the moment. Objective: The aim of this study is to build up a simple screening procedure for beta-coronavirus (MERS-CoV). Material and method: Synthetic RNA pieces derived from the archived genome of betacoronarivus (MERS-CoV) provided by the European Virus Archive (EVA), Invitrogene vector NTI-11.2 software and bispies recruited from Tran Hung Dao University Hospital. Reverse transcriptase followed by polymerase chain reactions (PCR): Result: The two primer sets of SHPT108ERS-CoV-L, SHPT108ERS-CoV-S were optimized to allow to detect the presence of one betacoronavirus RNA copy out of 1,000 ABL RNA copies without any mis-amplification from MERS-CoV free samples. Conclusion: The simple screening procedure for beta-coronavirus (MERS-CoV) was successfully designed.