Xây dựng quy trình phân tích heroin, cocain và các sản phẩm chuyển hoá trong nước tiểu bằng GC/MS

A rapid, accurate and adequate GC/MS method for determination of heroin, cocaine and their transformed components in urine was developed and validated: Column: EVDX- 5MS (25m x 0.2mm x 0.33um); DeL EI MSD quadruple, ionization is touching to 70eV quadruple. The ion source temperature: 230°C. The ion line temperature: 280°C.; Inj. vol: 1ul; inj. temp. 250°C; carrier gas: helium; flow rate: 0.8ml/min. The temperature was programmed as follows: initial: 70°C for 2 min.; increased by 20oC/min. to 160°C, kept isotherm. (160°C) for 2 min.; then increased by 5oC/min. to 280°C. kept isotherm (280°C) to the end. The total temperature program time was 42.5 minutes. Samples were analyzed by two conditions. Scan and SIM. This GC/MS procedure was used to analyse 11 urine samples. The tested components were positive in these all samples.