Biểu hiện gen chịu trách nhiệm sinh tổng hợp hợp chất polyketid từ loại bọ Paederus fuscipes

Based on analysis of the complete sequence of the small PKS-NRPS cluster not belonging to pederin cluster isolated from the genome of the symbiont, the structure of the unknown polyketide was predicted. By RED-mediated homologous recombination, this PKS-NRPS gene cluster of about 37.5 kb in size located on the cosmid pPD7E4 was introduced successfully into the expression vector pJB861 and then transformed into the host cell P. putida KT2440. Plasmid DNA from the recombinant strain P. putida was checked by restriction analysis. mRNAs from P. putida that were transcribed from the entire PKS-NRPS gene cluster were identified by RT-PCR. Up to this step, the microbial expression system including the expression vector pJB861 and the host cell P. putida KT2440 was recognized to be an effective tool to express PKS-NRPS genes. Therefore, to obtain the target compound for structural characterization and bioassay requires further investigation.