Using indirect ELISA IgM technique detected IgM anti Banna virus from 717 cerebrospinal fluid samples of patients who has acute encephalitis syndrom unknown causes were collected from 9 provinces/cities in the North, Central, South and Highland Central from 2002 to 2012. Positive results identified the Banna virus antigen average is 184/717 (25.66 percent). On these results, sero-epidemiological characteristics of acute encephalitis syndrome virus Banna is determined as follows: (1) Propotion positive was ranged from 13.63 percent - 35.83 percent of the 9 provinces/cities, where samples were collected for the detection of IgM against Banna virus; (2) The positive cases were recorded all year round, but mainly in summer months May, June and July with highest prositive cases in June to be 34.78 percent; (3) The distribution positive cases by age groups were different age group, the lowest in age group I year old was 5.98 percent, the highest in age group or = 15 year olds was 28.26 percent, other age groups ranged 19.02 percent - 23.91 percent.
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