
Thông tin nghiên cứu
Loại tài liệu
Bài báo trên tạp chí khoa học (Journal Article)
Tiêu đề
Nghiên cứu phát triển phương pháp Reverse Transcription-Loop Mediated Isothermal Amplification (RT-LAMP) phục vụ định typ virus Dengue
Tác giả
Trương Quốc Phong
Năm xuất bản
2014
Số tạp chí
102
Trang bắt đầu
154-157
ISSN
0868-3980
Từ khóa nghiên cứu
Abstract
With the purpose of development of the method for rapid detection and typing Dengue virus, the research group has successfully developed the RT-LAMP method for detection of Dengue virus from patient serum. In the present study, the RT-LAMP method has also established for determining 3 types of Dengue virus (type 2, 3 and 4). This assay is very simple and rapid; RT-LAMP products could be detected after 60 min incubation under isothermal condition at 61°C. The established RT-LAMP assay was tested for typing of Dengue virus in patient serum samples. These findings indicate that the RT-LAMP assay has the potential application for rapid detection and typing of Dengue virus from patient serum and suitable for laboratories with simple equipments.
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