Nghiên cứu sản xuất thử nghiệm mẫu máu giả định đông khô với tác nhân gây bệnh nhiễm khuẩn huyết

This is and experimental research for production of artificial blood samples containing Streptococcus pneumoniae, Haemophilus influenzae cells by freeze-drying method with using sucrose 5% and human serum 20% as lyoprotectant to preserve bacteria. The empirical research method was used for this research. The one-way analysis of variance (ANOVA) is used to assess the homogeneity while T-test is used to assess stability at two different temperature ranges- at 22 - 30°C after 1, 3, 5, 7, 9, 12 weeks and at 2 - 8°C after 1, 2, 3, 4, 5, 6 months. Through the assessment of homogeneity, the Log average concentration of S. pneumoniae, H. influenzae cells in artificial blood samples were 5.610 ± 0.031 CFU/mL and 5.533 ± 0.022 CFU/mL (Fexperimental < Ftheory và p > 0.05). By evaluating stability, at 22 - 30°C, SP samples were stable after 7 weeks (p = 0.091) and HI samples were stable after 3 weeks (p = 0.073); while at 2 - 8°C, SP samples were stable after 5 months (p = 0.051) and HI samples were stable after 3 months (p = 0.051). Through this research, we demonstrated that two sets of artificial blood samples containing S. pneumoniae and H. influenzae met the quality standards of ISO 13528 and ISO Guide 35.