
For rapid detection of Staphylococcus aureus causing Staphylococcal scalded skin syndrome (SSSS), a PCR test with primers was applied specific to genes encoding exfoliative toxins (ETA and ETB). DNA template used in the PCR reaction was extracted from 24 strains of S. aureus isolated from patients with SSSS admitted to the National Hospital of Dermatology and Venerelogy. Results of PCR showed that all strains have both genes coding for toxins ETA and ETB. The rate of having the gene in female patients was 1.5 times higher than men. In addition, more than 83 percent of patients aged 3 were seen. Sequencing results showed the nucleotide sequences of the genes coding for ETA and ETB were 100 percent similar to those in the international gene bank.
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