Nghiên cứu phát triển phương pháp Reverse Transcription-Loop Mediated Isothermal Amplification (RT-LAMP) phục vụ định typ virus Dengue

With the purpose of development of the method for rapid detection and typing Dengue virus, the research group has successfully developed the RT-LAMP method for detection of Dengue virus from patient serum. In the present study, the RT-LAMP method has also established for determining 3 types of Dengue virus (type 2, 3 and 4). This assay is very simple and rapid; RT-LAMP products could be detected after 60 min incubation under isothermal condition at 61°C. The established RT-LAMP assay was tested for typing of Dengue virus in patient serum samples. These findings indicate that the RT-LAMP assay has the potential application for rapid detection and typing of Dengue virus from patient serum and suitable for laboratories with simple equipments.