Aims: To establish a quick protocol, which can work complementarily to conventional - microbial blood - culture approach. Material and methods: Colonies of Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, Streptococcus pneumonia, and Candida albicans were isolated at the Department of Microbiology used as positive control to monitor the success of individual test. Primer pairs are designed in genus - specific - manner, that devoid of cross (or/and) mis-amplifying human DNA template. The specificity of primer pair is confirmed by Sanger sequencing, primer amplifiable sensitivity was assayed using CFU dilution series. Result: Optimized conditions for PeR had been set up, that could detect 7 pathogens such as C. albicans, P. aeruginosa, S. aureus, A. baumannii, E. Coli, S. Pneumonia and K. pneumonia, when the pathogens' load exceeds a thresold 1-100 CFU/ml. Condusion: A molecular approach for identification of blood sepsis related pathogens was launched with a high sensitivity and specificity.
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