Background: Cryopreserved-thawed embryos have a lower rate of pregnancy than fresh embryos, which might be due to the hardening of the zona pellucida of frozen embryos. The aim of this study is to assess the effectiveness of assisted hatching on implantation as well as clinical pregnancy rates after the transfer of frozen-thawed embryos. Methods: The study was performed on 98 patients in the Centre of Human Reproduction and Embryology (Institute of Military Medicine), with 257 cryopreserved embryos on day 3 applying the vitrification technique. Results: It is obvioulsy shown that patients with assisted hatching for embryos have higher rates of implantation and clinical pregnancy than those without assisted hatching for embryos (16.26 percent versus 7.14 percent and 33.33 percent versus 15.9 percent respectively. Conclusion(s): Assisted hatching technique using acidic tyrode can increase the implantation rate in cryopreserved-thawed embryos (p 0.05).
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