
The purpose of this study was to assess the rate of survival of frozen epididymis sperms after being thawed and to evaluate the effectiveness of the method of injecting frozen - thawed epididymis sperm into intra - cytoplasm in. Clinical interventional trial: of 89 couples whose epididymis sperm was frozen - thawed, 79 cases had enough survival sperm for ICSI representing approximately 88.8 percent; there are 10 cases of 100 percent sperm dead representing approximately 11.2 percent; in 72 cases whose embryos were transferred, there were 43 clinical pregnancy cases representing approximately 59.7 percent, 7 cases whose embryos were totally frozen because of ovarian hyper - stimulation syndrome. The preliminary results on applying technique of injecting frozen - thawed epididymis sperm into intra - cytoplasm have shown that this method was effective and there were no difference comparing to the method of using fresh epididymis sperm.
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