Kết quả phát hiện Mycobacteria tuberculosis bằng xét nghiệm 16S rRNA-real-time ExBP-RT-PCR ở bệnh nhân lao phổi mới AFB âm tính

To evaluate the results of the the results of detection of Mycobacteria tuberculosis in bronchial lavage fluid by 16S rRNA-real-time ExBP-RT-PCR test in patients with newly diagnosed pulmonary tuberculosis AFB smear-negative. Subjects and methods: A prospective, descriptive, cross-sectional study was conducted on 30 patients with newly diagnosed pulmonary tuberculosis AFB smear-negative and 30 community-acquired pneumonia ones, treated as inpatient at the Respiratory Center, Military Hospital 103, from September 2021 to January 2024. Evaluating the sensitivity and specificity of the 16S rRNA-real-time ExBP-RT-PCR test in detection of Mycobacteria tuberculosis, comparing with the results of the GeneXpert/Mtb-Rif test. Results: Results of 16S rRNA-real-time ExBP-RT-PCR test were positive in 25 patients (83,3%). The sensitivity and specificity of the test follow 83.3% and 100%, respectively. The positive proportion of the 16S rRNA-real-time ExBP-RT-PCR test is higher than GeneXpert/Mtb-Rif but not statistically significant (p > 0.05). Conclusions: Intitial result of 16S rRNA-real-time ExBP-RT-PCR test has high sensitivity, specificity in detection of Mycobacteria tuberculosis in patients with newly diagnosed pulmonary tuberculosis AFB smear-negative.