Human papillomavirus (HPV) is an important cause leading to cervical cancer and is a common sexually transmitted disease especially in women. Polymerase chain reaction (PCR) methods to detect HPV is highly sensitive and widely used. Objective: To compare GP5 + /GP6 + original primers (GP5 + / GP6 + original-PCR) and GP5 + / GP6 + modified primer (GP5 + I GP6 + modifile-PCR) to detect HPV DNA from cervical swabs of 481 female sex workers in Hai Phong, Vietnam. Methods: (1) Collect the sample in 481 female sex workers in Hai Phong, Vietnam (2) Detection of HPV DNA by PCR with two primers GP5 + / GP6 + original and GP5 + I GP6 + modified, (3) HPV typing using DNA chip (DNA micoaray) and clonal sequencing. Results: With PCR using GP5 + / GP6 + original primers. the prevalence of HPV-positive was 50.1 percent (241/481) and 49.6 percent (236/481) using primers GP5 + / GP6 + modified (GP5 + / GP6 + modifile-PCR). Among 241 samples of HPV DNA GP5 + / GP6 + original-PCR positive, 237 samples have been determined with 29 HPV different genotypes were detected. From the 236 samples of HPV DNA GP5 + / GP6 + modiflie-PCR positive. 100 percent of the samples were HPV type detected with the same results with GP5 + / GP6 + original-PCR possitive samples. Conclusions: Both GP5 + / GP6 + original and GP5+/ GP6 + modifile primer sets amplified a wide spectrum of HPV genotypes in cervicovaginal samples and detected similar prevalences of HPV positivity. However, GP5 + / GP6 + original primers have a higher sensitivity but lower specificity than GP5 + / GP6 + modifile primers.
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