The factors of cardiac risk in patients with type 2 diabetes mellitus had known. However, they do not explain the increased cardiovascular related morbidity and mortality in these patients. Fibrinogen may have a role in this excess risk. Fibrinogen (factor I) is a soluble plasma glycoprotein, synthesized by the liver, that is converted by thrombin into fibrin during blood coagulation. The role of fibrinogen has been recently better understood. Reports have suggested that fibrinogen is related to type 2 diabetes mellitus and its complication as cardiovascular diseases. In this study, affinity chromatography with lectin concanavalin A (ConA) was used to obtain glycoproteins from serum samples. Then, glycoproteins were separated in 2-D electrophoresis and gels with protein spots were cut and digested by trypsin. The peptide mixtures extracted from each gel slice were analysed by one-dimensional nano liquid chromatography (I DnanoLC) coupled online with tandem mass srt:ctrometry analysis (NanoESI-Q-TOF-MS/MS). The proteins were identified by Mascot vl.8 software and prJcessed by MSQuant v1.5 software for further validation. These proteins were characterized based on their role according to Gene Ontology using bioinformatics tools. It was shown that fibrinogen was in the excess in the serum of type 2 diabetes mellitus patients. Further research will be focused on the relationship of higher expression of fibrinogen and its role in type 2 diabetes mellitus and cardiovascular diseases.
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